Description of the Rare Marine Ciliate, Uronychia multicirrus Song, 1997 (Ciliophora; Euplotida) based on Morphology, Morphogenesis and SS rRNA Gene Sequence

ABSTRACT. A population of the marine euplotid ciliate, Uronychia multicirru s Song 1997 , found in the littoral zone of the Daya Bay, Guangdong, South China, was investigated using live observation and protargol impregnation method. This species is diagnosed by possessing a long row of ventral cirri, which has never been seen in all other known congeners. Divisional process, described based on protargol-impregnated specimens, is typical of the genus regarding its general pattern: (1) the oral primordia in both proter and opisthe develop de novo in two subcortical pouches, while the UM-anlage forms de novo (i.e. does not develop from the oral primordium); (2) five frontoventral-transverse (FVT)-cirral anlagen develop in a primary-mode on the cell surface, and almost all the cirri are derived from the FVT-anlagen, except the first frontal cirri, which are formed de novo in both dividers; (3) the species characteristic ventral row derives from the right-most FVT-anlage; (4) the parental structures do not take part in the construction of the marginal anlagen, and (5) the caudal cirri originate in the right-most two dorsal kineties in a multi-segmentation mode. The small subunit rRNA gene of U. multicirrus (GenBank accession number: EU267929) is 1,773 bp and differs by 0.9–1.41% from its closely related congeners.     

Ptk2b deletion improves mice folliculogenesis and fecundity via inhibiting follicle loss mediated by Erk pathway

Ptk2b has been found playing critical roles in oocyte maturation and subsequent fertilization in vitro. But what is the exact in vivo function in reproduction still elusive. Here, by constructing Ptk2b mutant mice, we found Ptk2b was not essential for mice fertility, unexpectedly, contrary to previously reported in vitro findings, we found Ptk2b ablation significantly improved female fecundity. Follicle counting indicated that the number of primordial follicles and growing follicles in matured mice was significantly increased in the absence of Ptk2b, whereas the primordial follicle formation showed no defects. We also found this regulation was in an autophosphorylation independent pathway, as autophosphorylation site mutant mice (PTK2B^Y402F) show no phenotype in female fertility. Further biochemistry studies revealed that Ptk2b ablation promotes folliculogenesis via Erk pathway mediate follicle survival. Together, we found a novel biological function of Ptk2b in folliculogenesis, which could be potentially used as a therapeutic target for corresponding infertility.     

凝胶电泳微流控芯片提取外泌体及对人血浆外泌体中miRNA-21的测定

为了开发一种用于人体血浆中外泌体的高效快速提取和分离的新型微流控芯片,文中收集健康人体外周血液样本,自主设计并制备基于纳米多孔薄膜和琼脂糖凝胶电泳的微流芯片。提取的外泌体使用透射电镜、Nanosight和Western blotting等技术进行表征,鉴定并分析其形态、浓度和粒径分布。同时将超速离心法和微流芯片所提取的外泌体进行粒径和浓度的分析比较,探讨两种方法各自的提取效率。最后,利用RT-PCR技术分析外泌体中miRNA-21的相对表达量。凝胶电泳微流芯片可在1 h内快速的从血浆中高效率地提取出纯度高、大小完整、尺寸分布在30–200 nm之间的外泌体,满足后续下游分析的要求。通过与现有最普遍的超速离心法进行对比分析,当血浆样本量小于100μL时,凝胶电泳微流芯片提取外泌体的效率为超速离心法的3.80倍。凝胶电泳微流芯片提取外泌体的优化参数是:电场电压:100 V;琼脂糖凝胶浓度:1.0%;注射泵流速:0.1 mL/h。凝胶电泳微流芯片可快速高效地提取出外泌体,对外泌体与癌症生物标记物的相关研究具有潜在的巨大优势,也为基于外泌体的即时诊断技术提供了可能。     

FcγR基因多态性与牙周炎易感性的Meta分析

目的探讨FcγRⅡa、FcγⅢa和FcγRⅢb的基因多态性与牙周炎发病风险性的相关性。方法通过检索PubMed、Web of Science、中国知网和万方等中英文数据库,纳入2019年10月前所有符合纳入标准的有关FcγRⅡa、FcγⅢa和FcγRⅢb的基因多态性与牙周炎发病风险性的研究,共27项病例对照研究,其中包含2 105个病例和1 115个对照,采用RevMan 5.3软件进行Meta分析,利用优势比(OR)及95%置信区间(CI)评价效应强度。结果 Meta分析结果合并显示FcγRⅡa和FcγRⅢb的基因多态性与牙周炎的发病风险无显著相关性,但FcγⅢa的基因多态性可能增加慢性牙周炎发病风险(V vs. F,OR=1.93,95%CI 1.01～3.69)。根据种群进行亚组分析,FcγRⅡa H131R位点的突变型可能会增加亚洲人群慢性或侵袭性牙周炎的发病风险[慢性牙周炎:R vs. H,OR=1.22,95%CI 1.04～1.42,(HR+RR)vs. HH,OR=1.28,95%CI 1.03～1.59;侵袭性牙周炎:R vs. H,OR=1.60,95%CI 1.01～2.54],但可能会降低高加索人群慢性牙周炎的发病风险[(HR+RR)vs. HH,OR=0.66,95%CI 0.48～0.90]。FcγⅢa F158V位点的突变型可能增加高加索人群慢性牙周炎的发病风险[V vs. F,OR=1.73,95%CI 1.06～2.85,(VV+FV)vs. FF,OR=2.26,95%CI 1.06～4.82]。FcγRⅢb NA1/NA2位点的NA2等位基因可能会降低包含50%高加索人群的混合人群侵袭性牙周炎的发病风险[(NA1NA2+NA2NA2)vs. NA1NA1,OR=0.57,95%CI 0.34～0.94)]。结论 FcγRⅡa、FcγⅢa和FcγRⅢb的基因多态性与牙周炎发病风险性之间的联系可能存在着种族差异,需要更大样本、更高质量的研究来进一步证实。     

荒漠油蒿（Artemisia ordosica）根围AM真菌分布与土壤酶活性

于2007年10月在油蒿(Artemisia ordosica)集中分布区选取4个典型样地,分别从0～10cm、10～20cm、20～30cm、30～40cm和40～50cm土层采集根围土样,分离其丛枝菌根(arbuscular mycorrhiza,AM)真菌并测定了3种土壤酶活性.结果表明,在各样地0～50cm土层中油蒿根系AM真菌菌丝定殖率均很高,有典型的泡囊与丛枝结构.菌丝定殖率与泡囊定殖率呈显著正相关,但菌丝、泡囊和丛枝定殖率与AM真菌孢子密度和土壤酶活性之间都无相关性;孢子密度在不同样地及采样深度间差异明显,与土壤有机质、速效磷和速效氮含量都呈一定正相关关系.孢子密度与脲酶和酸性磷酸酶活性有显著或极显著相关关系,与碱性磷酸酶活性之间的相关性受到土壤pH的显著影响.孢子密度的峰值出现在0～10cm表层土,并随土壤剖面深度增加而降低.土壤酶活性在土壤垂直剖面显示与孢子密度同样的规律.不同样地间AM真菌分布及油蒿根系定殖率的差异表明,油蒿与AM真菌之间有良好共生性,对维护荒漠生态环境系统结构的完整性具有重要意义.     

旋转磁场联合5-FU对SP2/0细胞超微弱发光强度的影响

目的:研究旋转磁场(RMF)联合氟尿嘧啶(5-Fu)对小鼠骨髓瘤细胞(SP2/0)超微弱发光的影响,探讨两者联合对肿瘤细胞的杀伤作用.方法:SP2/0细胞分为四组:对照组,单纯5-FU化疗组(单化组),单纯磁疗组(单磁组),磁场联合5-FU组(磁化组).用RMF、5-FU作用于SP2/0细胞,化学发光仪进行分析.结果:对照组的化学发光强度值最低,单化组、单磁组、磁化组化学发光强度值均明显高于对照组(P均<0.001).单磁组低于单化组及磁化组,有明显差别(P<0.001);磁化组的化学发光强度值最高,高于单化组(P<0.001).结论:磁场和5-Fu均可引起SP2/0细胞化学发光强度值的变化,以磁场联合5-Fu的化学发光强度值变化最明显,提示磁场单独及联合5-FU对SP2/0细胞有明显的杀伤作用.其机理可能与旋转磁场和5-FU引起细胞自由基增高有关.     

Event-related potentials in a Go/Nogo task of abnormal response inhibition in heroin addicts

Inhibitory control dysfunction is regarded as a core feature in addicts. The major objective of this study was to explore the time course of response inhibition in chronic heroin addicts and provide the neu-rophysiological evidence of their inhibitory control dysfunction. The amplitudes and latencies of ERP components were studied in fourteen heroin addicts (mean duration of heroin use being (13.54±5.71) years (Mean±SD), average abstinence being ((4.67±6.44) months)) and fourteen matched healthy con-trols with a visual Go/Nogo task. Our results showed that heroin addicts demonstrated significantly larger Go-N2 amplitudes which results in a decreased N2 Go/Nogo effect, but no statistically significant differences were found between heroin addicts and controls in P3. The ERP data suggest that fronto-central areas of heroin addicts were impaired during the inhibition process (200—300 ms) and over-activated to targets. The impaired early process might reflect an abnormal conflict monitoring process in heroin addicts. These results consolidate the inhibitory control dysfunction hypothesis in chronic heroin users.     

贵州黔西县少数民族ABO血型分布及基因频率调查

对贵州黔西县1260例6个少数民族人群红细胞ABO表现型进行了检测;结果如下:贵州黔西县布依族、满族、苗族、白族这四个民族的ABO血型基因频率很相近,彝族和仡佬族与这4个民族的差别较大,布依族,苗族,满族,白族ABO血型分布为O>B>A>AB,彝族为O>A>B>AB,仡佬族为A>O>B>AB;经Hardy Weinberg吻合度检测可以证明贵州黔西县的ABO血型表现型分布状况及基因频率相对稳定,其分布符合hardy Weinberg平衡,获得了该地ABO血型系统群体遗传学数据,为群体遗传学的研究提供了一定的资料。     

针对SARS冠状病毒重要蛋白的siRNA设计(英文)

NA干涉 (RNAinterference ,RNAi)是一种特异性地导致转录后基因沉默的现象 ,在哺乳动物细胞中小分子干扰RNA双链体 (smallinterferingRNAduplexes ,siRNAduplexes)可以有效地诱导RNAi现象 ,为一些疾病的治疗开辟了新的途径 .针对SARS冠状病毒 (SARScoronavirus ,SARS CoV)中编码 5个主要蛋白质的基因 ,用生物信息学的方法设计了3 48条候选siRNA靶标 .在理论上 ,相应的siRNA双链体能特异地抑制SARS CoV靶基因的表达 ,同时不会影响人体细胞基因的正常表达 ,这为进一步siRNA类药物的实验研究提供了理论基础